Crystallization is a major method to let us find out the protein structure! Accroding to protein crystal diffraction, the conformation of it can be seen in real. However, it has been forced to arrange together so that the form might be not the real in nature!
Recently, 2D crystallization can help us to see the protein structure in alomst natural condition. And also the protein which has lots of subunits can been seen how they assemble especially membrane protein!
The picture shows you the comformation of 2D crystallization under EM observation!
After seeing the crystallization, software can help us to make all parts to be built perfectly!
![](//photos1.blogger.com/blogger/731/1792/400/figvis1.jpg)
Combine traditional and new 2D crystallization methods, we can understand how the protein works and the mechanism of it!